PROTEIN CHROMATOGRAPHY PRODUCTS
Micro Flow Through pH Monitor
The Acu I Low Cost-High Performance Conductivity Monitor
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MICRO FLOW THROUGH pH MONITOR
Low dead volume system designed specifically for protein chromatography
applications
Connects directly to your WatersTM 650 Advanced Protein Purification System
You can now optimize your protein separations by continuously monitoring
buffer pH
Features
*INTEGRATED SYSTEM. The Micro Flow Through pH Monitor comes complete with a
unique flow-through cell assembly, pH electrode, tubing and fittings for
fluid connections and a cable to connect the electrode to your existing pH
meter. An optional pH meter provides a digital display of pH and facilitates
transmission of the pH output to a recorder or automated data collection
system.
*LOW DEAD VOLUME. Flow through pH electrode has an internal volume of less
than 50 microliters for low flow rate cell and less than 150 microliters for
high volume flow cell.
*REAL TIME DIGITAL pH READINGS. pH meter gives digital display of pH of
effluent as it enters flow cell.
*CONTINUOUS pH RECORDING. The micro flow through pH electrode can be hooked
up through pH meter to a strip chart recorder for continuous pH monitoring
and recording.
*EASY INTERFACE WITH WATERS 650 APPS SYSTEM. Fluid connections are
straightforward, using fittings provided in the 650 APPS start-up kit.
*FLOW CELL OPTIONS ACCOMMODATE 650 APPS SYSTEM FLOW RATE OPTIONS. The
standard flow cell is suitable for use with 650 APPS systems configured to
operate at flow rates up to 45 ml/min. For systems equipped for 80 ml/min
maximum flow rates, an optional larger volume flow cell is available.
APPLICATION AREAS
*Continuously monitor impact of elution buffer pH manipulations on the
resolution of a complex protein mixture by ion exchange, hydrophobic
interaction or affinity chromatography.
*Take full advantage of Waters unique Auto-BlendTM capability for
automatically adjusting elution buffer pH for unattended method optimization.
PRODUCT SELECTION GUIDE
Flow Rate Electrode Assembly
0-45 ml/min FTPH-2
0-80 ml/min FTPH-2HF
ORDERING INFORMATION
Part Name Part Number
Model FTPH-2 Electrode Assembly FTPH-2
includes 50µl flow cell, pH electrode, tubing
and fittings for fluid connections, and BNC
cable
Model FTPH-2HF Electrode Assembly FTPH-2HF
includes 150µl flow cell, pH electrode, tubing
and fittings for fluid connections, and BNC
cable
Model MP-12 mV/pH Meter 110V MP-12
220V MP-12-220
includes recorder output cable
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ACU I LOW COST- HIGH PERFORMANCE CONDUCTIVITY MONITOR
Indispensable tool for ION exchange separations of proteins
Connects Directly to Your WatersTM 650 Advanced Protein Purification System
You can now optimize the salt gradient separation of proteins by continuously
monitoring the conductivity of your ion exchange buffer during your run
Features
*INTEGRATED SYSTEM. The Acu I Conductivity Monitor comes complete with a
unique flow-through conductivity cell assembly, electronic monitoring system
with optional digital display, and all fluid lines and electrical cables
required.
*INDISPENSABLE TOOL FOR LC GRADIENT ELUTION OF PROTEINS. The conductivity
monitor is ideally suited for continuous monitoring of salt gradients in ion
exchange, reverse phase, hydrophobic interaction, and hydroxylapetite
separations.
*ULTRASTABLE SOLID STATE ELECTRONICS. The conductivity monitor uses the latest
state of the art integrated circuitry, giving baseline stability, signal to
noise ratio, and overall performance comparable to units costing many times
as much.
*ELECTRONIC ZERO SUPPRESSION. The conductivity monitor can be adjusted from
0 to 200% zero suppression using a panel mounted precision 10 turn
potentiometer.
*BROAD CONDUCTIVITY RANGES. The conductivity monitor has 3 front panel
selectable conductivity ranges: 0 to 100, 0 to 1000, and 0 to 10,000
microsiemens. A high range monitor up to 100,000 microsiemens is available.
*EASY INTERFACE WITH WATERS 650 APPS SYSTEM. Fluid connections are
straightforward, using fittings provided in the 650 APPS start-up kit.
*FLOW CELL OPTIONS ACCOMODATE 650 APPS SYSTEM FLOW RATE OPTIONS. The standard
flow cell is suitable for use with 650 APPS systems configured to operate at
flow rates up to 45 ml/min. For systems equipped for 80 ml/min maximum flow
rates an optional larger volume flow cell is available.
*LOW VOLUME FLOW CELL. The conductivity cell has an internal volume of less
than 20 microliters.
*POSITIVE TEMPERATURE CONTROL. The conductivity monitor incorporates a
thermister in the flow cell for positive temperature compensation.
*IDEAL FOR LOW COST METHODS DEVELOPMENT. The conductivity monitor is ideal
for low cost chromatography methods development for a wide variety of
samples using different columns.
APPLICATION AREAS
*ELIMINATE GUESSWORK IN PROTEIN SEPARATIONS. Determine exact buffer
compositions at the point that each protein elutes from the LC column.
*OPTIMIZE DESALTING. Determine optimal cutoff point of separation of salt
from protein in gel filtration.
*MINIMIZE REGENERATION TIME OF ION EXCHANGE COLUMNS. Minimize the time to
equilibrate an ion exchange column after a separation completion.
*VERIFY COLUMN PACKING EFFICIENCY. Determine LC column packing efficiency
before proceeding with a separation run.
PRODUCT SELECTION GUIDE
Conductivity Range
Flow Rate 0-10,000 0-100,000
Microsiemens Microsiemens
0-45 ml/min ICM-180 ICM-180HC
0-80 ml/min ICM-180HF ICM-180HCF
ORDERING INFORMATION
Part Name Part Number
Acu I Model ICM-180, 110V ICM-180
220V ICM-180-220
includes 20µl flow cell, monitor with three user selectable
conductivity ranges up to 10,000 microsiemens & cables
Acu I Model ICM-180HC, 110V ICM-180HC
220V ICM-180HC-220
includes 35µl flow cell, monitor with three user selectable
conductivity ranges up to 100,000 microsiemens & cables
Acu I Model ICM-180HF, 110V ICM180HF
220V ICM-180HF-220
includes 100µl flow cell, monitor with three user selectable
conductivity ranges up to 10,000 microsiemens & cables
Acu I Model ICM-180HCF, 110V ICM-180HCF
220V ICM-180HCF-220
includes 100µl flow cell, monitor with three user selectable
conductivity ranges up to 100,000 microsiemens & cables
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