Isoelectric Focusing pH Measurement
Isoelectric Focusing pH Electrode
Isoelectric
Focusing pH profiling system
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Isoelectric Focusing pH Electrode
The Model
1210 is a specially constructed Flat Combination Electrode
designed
especially for isoelectric focusing techniques. This
versatile electrode
is suitable for determination of pH gradients and
isoelectric points in
thin layer granular gels, preparative gels,
flat bed polyacrylamide gels,
tube and disk gels and gel fractions.
This unique sensing end measures less
the 6 1/2mm in diameter and is
constructed with an annular reference
junction concentric and flush
with the flat pH sensing tip. Because of
this small sensing area,
adjacent isoelectric points can be differentiated
by at least 0.02 pH
units. pH values and isoelectric focusing points for
0.5 to over
10mm thick layers are accurately measured using the
special
electrode. Compatible with most pH meters. 145mm overall
length,
6 1/2mm sensing tip.
Features:
* Miniature Flat Sensing Area
*
Less Than 6 1/2mm in Diameter
* Calomel Reference
* Ceramic Reference
Junction
Specifications:
pH Range: - 14 pH
Temperature Range: 5 to
60C
Dimensions: Length 145mm
Tip O.D. 6 1/2mm
Note: When ordering,
specify type of pH meter or plug.
Ordering Information
Model 1210
Isoelectric Focusing pH Electrode
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Isoelectric Focusing pH Profiling System
Determine pH gradients and
isoelectric points in
* thin layer granular gels
* flat bed
polyacrylamide gels
* preparative gels
* tube gels
* disc gels
Unique
Miniature pH Sensor. The Model 1210 pH probe features a
miniature flat pH
sensor in combination with an annular reference
junction especially
designed for pH measurements of isoelectric gels.
Accurate Gel pH
Measurements. Because of the pH sensors small
sensing area adjacent
isoelectric points can be differentiated by at
least 0.02 pH units.
Use
with Both Flat Slab and Tube Gels. The Lazar pH Profiling System
can
easily be used with both flat slab and tube gels.
Direct Correlation
Between Protein Banding & pH Profile. Using the
Lazar pH Profiling
System, a direct correlation can be obtained
between protein banding
patterns and pH profile without gel slicing.
Compatible with most pH
meters. The Lazar pH Profiling System can be
used with most laboratory
type pH meters. The Lazar Isoelectric pH
Electrode plugs directly into pH
meter input.
Avoids Tedious Chemical pH Markers and Gel Sectioning. The
Lazar pH
Profiling System gives accurate direct pH measurements on the
surface
of gels eliminating the need for get sectioning or chemical pH
markers.
Ordering Information
Complete pH Profiling System. Model ISO
includes ISO-276 gel
positioning system pH7 buffer and Model 1210
miniature flab pH
electrode.
Order Cat. No. ISO-376
pH Probe. Model 1210
Miniature Flat pH Probe with 30 inch cable and
U.S. Standard Connector
(Radiometer), B.N.C. and other connectors
also available.
Gel Positioning
System. Model ISO-276 includes electrode manipulator
arm, electrode
holder, precision machined sample hold block for
holding and positioning
tube gels, precision machined plexiglass tray
for holding flat slab gels
and fluoropolymer spacers for securing tube gels.
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