Real Time Monitoring of pH, pO2 & CO2 Optimizes Cell Culture
and Organ Perfusion Studies
One of the major trends in biotechnology today is the
increasing use of mammalian cell lines including human,
monkey, mouse and bovine cells. Various types of bioreactors
are now being used to culture these animal cells. These
include well plates, T-flasks, stirred vessels, spinner
flasks, hollow fiber and air lift bioreactors. One of the most
important requirement for optimal cell growth in a bioreactor
is continuous monitoring of critical parameters which include
pO2, pH, CO2, and temperature.
These same parameters are also critical for conducting organ
perfusion studies on the isolated rat liver or other organs of
various animals such as heart, lung and kidney.
Lazar Research Laboratories, Inc. has recently introduced a
series of micro flow through electrodes which are ideal for
monitoring bioreactors and organ perfusion work. These
electrodes include a micro flow through model for pH (Model
FTPH-2), pO2 (Model DO166-FT) and pCO2 (Model GS-136-COFT).
The pH and pCO2 electrodes can be directly attached to any
laboratory pH meter. The output from the pH meter can be fed
to a strip chart recorder for continuous monitoring. The pO2
probe can be directly interfaced with either a pH meter or a
strip chart recorder.
These low cost electrodes (under $500) each) attach directly
to standard size rubber or plastic tubing used in bioreactors
and organ perfusion systems. The electrodes offer the
scientist on a limited budget a modular cost effective
approach to real time monitoring of their bioreactor or
perfusion experiments. A micro temperature probe (Model T-
176M) which utilizes a tiny thermister bead is also available
for continuous temperature monitoring directly in the
bioreactor or at the organ site.
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