Real Time Monitoring of pH, pO2 & CO2 Optimizes Cell Culture and Organ Perfusion Studies
One of the major trends in biotechnology today is the increasing use of mammalian cell lines including human, monkey, mouse and bovine cells. Various types of bioreactors are now being used to culture these animal cells. These include well plates, T-flasks, stirred vessels, spinner flasks, hollow fiber and air lift bioreactors. One of the most important requirement for optimal cell growth in a bioreactor is continuous monitoring of critical parameters which include pO2, pH, CO2, and temperature. These same parameters are also critical for conducting organ perfusion studies on the isolated rat liver or other organs of various animals such as heart, lung and kidney. Lazar Research Laboratories, Inc. has recently introduced a series of micro flow through electrodes which are ideal for monitoring bioreactors and organ perfusion work. These electrodes include a micro flow through model for pH (Model FTPH-2), pO2 (Model DO166-FT) and pCO2 (Model GS-136-COFT). The pH and pCO2 electrodes can be directly attached to any laboratory pH meter. The output from the pH meter can be fed to a strip chart recorder for continuous monitoring. The pO2 probe can be directly interfaced with either a pH meter or a strip chart recorder. These low cost electrodes (under $500) each) attach directly to standard size rubber or plastic tubing used in bioreactors and organ perfusion systems. The electrodes offer the scientist on a limited budget a modular cost effective approach to real time monitoring of their bioreactor or perfusion experiments. A micro temperature probe (Model T- 176M) which utilizes a tiny thermister bead is also available for continuous temperature monitoring directly in the bioreactor or at the organ site.

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